Genome wide association study for stripe rust resistance in spring bread wheat (Triticum aestivum L.)

Abstract Stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ) is one of the most destructive diseases wheat Triticum aestivum L.) worldwide causing huge yield losses every year. Development and deployment resistant varieties economical environment friendly approach for controlling this disease. However, because continuous evolution pathogen, genes are easily overcome new virulent races, which necessitates a identification introgression resistance to develop varieties. To identify effective source resistance, genome-wide association study was performed using 426 elite bread genotypes based on 5176 polymorphic Diversity Arrays Technology (DArT) markers. Adult-plant-resistance evaluated under field conditions yellow rust two consecutive years (2014 2015) at ICARDA Merchouch station, Morocco. Out genotypes, 51.17% were highly with 5–10% level severity rust. Genome wide studies (GWAS) mixed linear model (MLM) identified three DArT markers chromosomes 1B, 2B 7B significantly associated stripe false discovery rate p ≤ 0.05. BLAST analysis confirmed that marker 412,394 in chromosome overlapped previously reported QTLs (QYrlu.cau-2BS1 Luke QYrid.ui-2B.1_IDO444). other 542,318 (1B) 583,038 (5B) not mapped within any gene/QTL regions; therefore, these may represent novel loci The linked molecular recommended further gene pyramiding purposes breeding programs after validation.